Project Objectives:

1. Evaluate amount and quality of human circulating RNAs and assess possible applications of epitranscriptomics profiling.
2. Optimise all steps of RNA extraction, chemical treatment and library preparation steps for reliable deep sequencing of target RNAs.
3. Establish technology allowing robust and precise epitranscriptomic profiling of RNA species present in human liquid biopsies and body fluids like blood, saliva and urine samples.
4. Apply the developed technology to profiling of the cohort of PCa cancer patients and compare them to healthy subjects.
5. Explore possibility for coupling this analysis to nanopore sequencing technology.

Your role:

1. Gain the knowledge on the composition of stable non-coding RNAs in human liquid biopsies and body fluids and select suitable and relevant targets for epitranscriptomic profiling.
2. Establish reference RNA modification profiles for healthy subjects.
3. Compare RNA modification profiles for healthy subjects and PCa patients.
4. Evaluate the suitability of RNA epitranscriptomic profiles as ONT biomarkers of PCa.

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