The proposed PhD project aims to:

1. Investigate the effect of Nm perturbation at selected specific ribosomal sites on cancer cell invasive behaviour by generating stable knockout and overexpression 2D, 3D cell lines and xenograft models.
2. Analyse the downstream molecular pathways by which specific Nm marks exercise their function to unveil their role in ribosome function and PCa progression.
3. Develop vectors targeting snoRNAs/Nm tandems relevant in PCa progression.

Expected results:

Perturbation of ribosomal 2’O-methylation (Nm) leads to deficient function of cancer ribosomes (translation) and our recent data supports the functional involvement of individual Nm marks in PCa tumour growth.

1. Generated molecular and cellular tools to study the effects of Nm perturbation on cancer cells.
2. Phenotypic characterization of the obtained cell models
3. Described the role of selected Nm marks in ribosomal function and their relevance in PCa progression.

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